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Month: June 2024

Owl Pellet Dissection

AYVA was recently given the opportunity to dissect different sizes of  Carolina’s owl pellets! Even with the limited stock, we were able to use both the small and large owl pellets to experiment on.

Owl pellets are remnants of an owl’s diet and digestive process, composed primarily of indigestible parts of prey such as bones, fur and feathers. These pellets are regurgitated several hours after an owl consumes its prey, and the process of regurgitation can last from seconds to several minutes. Young owls do not produce pellets until they have begun to eat their prey whole.

These pellets provided us with a fascinating look on what an owl’s regular feeding diet may look like. Within both pellets we found multiple bones relating to brown rats, one of the many food sources of everyday barn owl’s. These bones included the femur, humerus, tibia, fibula, jaw, skull and pelvic bone. These were easy to identify thanks to the owl pellet study kit. This kit contains a manual with plenty of pictures of all the bones that you might find within an owl pellet.

 

While we only dissected two owl pellets, each pellet can differ from the next, whether they are small or large. The shape and texture of a given owl pellet depends on the species owl and its prey. Pellets can be tightly compacted, oval, and furry, or loosely packed with an irregular shape.

As we compared the dissection of the larger and smaller pellet we noticed that there are more remains in the larger pellet. The larger owl pellet had more identifiable remnants of the prey making it easier to determine the owls’ feeding habits and local ecosystem. By using the larger pellets, students have more components to analyze to give a closer look into the owls behavior.

What Are Owl Pellets?

owl pellets

See what you can learn about birds by studying the pellets they leave behind.

Most birds cannot chew their food, and owls are no exception. Owls usually swallow their prey whole. Owls differ from other species of birds because they do not have a crop, the baglike organ used to store food after it has been swallowed so that it can be digested later. In owls, food passes directly from the mouth to the gizzard. The gizzard is an organ that uses digestive fluids and bits of sand and gravel to grind and dissolve usable tissue from the prey.

The types of tissue that can be dissolved by an owl’s digestive system include muscle, fat, skin, and internal organs. These tissues are broken down into a variety of nutritional substances by the owl’s gizzard and intestines. Some of these tissues (e.g., fur and bones) cannot be digested. The digestible material, along with other waste collected throughout the body, is ejected from the vent, which is the combination reproductive and excretory opening in birds. The pasty white excrement is known as urea. It is rich in nitrogen and similar to urine in mammals, only thicker.

What happens to the indigestible material?

Indigestible material left in the gizzard such as teeth, skulls, claws, and feathers are too dangerous to pass through the rest of the owl’s digestive tract. To safely excrete this material, the owl’s gizzard compacts it into a tight pellet that the owl regurgitates. The regurgitated pellets are known as owl pellets.

Owl pellets are useful to researchers because they can find out quite a bit about an owl’s lifestyle through careful examination of the pellet’s contents. Since most of the prey’s bones are not actually broken during the attack and the subsequent digestion process, they can be readily identified in the pellet. Most pellets include a skull or skulls, which makes identification of the prey relatively simple. If an owl consumes multiple prey in a short period of time, it forms one large pellet from the remains.

Large owls are obviously capable of making large pellets. However, since large owls do not always eat large prey, one cannot always determine the size of the owl that left a given pellet solely based on the size of the pellet. In addition, a startled owl may eject a pellet that is not fully compacted, thereby giving the pellet a larger appearance than normal. Other species of birds such as hawks and eagles produce pellets, but they are smaller and contain fewer animal parts than those produced by owls.

Skulls and other bones can be found during an owl pellet dissection.

Storing and regurgitating pellets

An owl pellet generally reaches its final form a few hours after the owl has eaten. However, the pellet is not usually ejected immediately after it is formed. Owls can store a pellet in a structure known as the proventriculus for as long as 20 hours before disgorging it. Since the stored pellet partially blocks the entrance to the digestive system, it must be ejected before the owl can eat again. Young owls do not produce pellets until they have begun to eat their prey whole.

The actual process of regurgitating a pellet lasts from a few seconds to several minutes. The pellet is forced out by spasms of the owl’s esophagus. These spasms make the owl look like it is coughing painfully. However, it is not hurt by the process because the pellet remains soft and moist until it leaves the owl’s body.

Identifying pellets

The shape and texture of a given owl pellet depends on the species of the owl that produced it and the type of prey that the owl consumed. Some pellets are tightly compacted, oval, and furry. Others are loosely compacted with an irregular shape. Pellets are moist when they are first ejected, but quickly dry out and start to decompose once they leave the owl’s body. Owl pellets are typically found near places where owls perch, such as under trees and near barns.

Barn Owl pellets are typically medium sized, smooth, cylindrical, and dark. The tiny Elf Owl has a very small pellet that is dry and loosely compacted, a result of its largely insect diet. The Great Horned Owl can produce pellets that are 3 to 4 inches long. These pellets are usually cylindrical and tightly compacted. The exterior of the pellet can vary greatly due to the vast array of prey that Great Horned Owls consume.

Owl pellet dissection resources

An owl pellet dissection gives students a glimpse into the life of an animal they may never see in the wild. Pellets tell us what the owl eats, where it is likely to roost, what small mammals live nearby, and even the relative proportions of those animals. Safe owl pellet dissections can build toward several NGSS standards across grade levels.

 

Frequently Asked Questions about Living Cultures

Many of you have questions about ordering and caring for cultures of protozoa, protists, and bacteria. We’ve gathered the most frequently asked questions here, along with the answers, as a quick reference. While it should answer most common questions and concerns, organism-specific information can be found in the instructions shipped with the culture and by searching our Carolina™ Care Sheets. Mitosis

Protozoa and protists

“When should I order protozoa?”

A living culture should be ordered a couple of days before use. We recommend receiving it on a Tuesday, Wednesday, or Thursday and using it the same week if at all possible. Try to arrange delivery at a time when you can personally receive the package and examine its contents. Inform your institution’s receiving department or office personnel to contact you immediately once it arrives. If you can’t get to the package right away, have someone place it in a climate-controlled area—NOT in a refrigerator—until you can attend to it.

“How long will my protozoa survive in the shipping container?”

Protozoa and algae cultures will survive for about one week in the shipping container. When you receive your protozoa culture, remove the lid and gently aerate the culture using a clean pipet. Allow the culture to settle after aeration and then use a stereomicroscope to check for activity. Replace the lid and set the culture in an area away from direct sunlight or extreme temperature changes.

  • For Euglena, make sure the cap is loose and place the container in a well-lit area, but not in direct sunlight.
  • As a rule of thumb, don’t place a protozoa, algae, or bacteria culture in the refrigerator. Exceptions are particular cultures of fungus that should be refrigerated to slow their growth, and when the instructions specify that refrigeration is an acceptable means of storage.

“How can I extend the life of my protozoa if I don’t want to subculture them?”

We recommend using the culture within 5 days of receipt. However, the life of your protozoa can be extended by feeding and by replenishing the culture water. For organisms that feed on bacteria, a single grain of precooked rice can be added to the culture container. Some organisms require algae or other food sources; be certain to research what food the organism requires prior to ordering. To replenish the culture water, allow the organisms to settle to the bottom of the jar, and then gently pour off about a quarter of the water and replace it with fresh springwater. Never use tap or distilled water.

“Some of the water from the culture container spilled (or evaporated) and I want to add more water. Can I just add some tap water? What kind of water is best?”

Never add tap water to a culture. It contains chlorine and in some cases chloramines that are toxic to protozoa, algae, and other living organisms such as fish and tadpoles. Use room temperature springwater instead. We recommend Carolina™ Springwater, which we use to grow many of our organisms. If this is not an option, you can purchase springwater from your local grocery store. Be sure to carefully read the label to ensure there are no additional additives for taste, and that the source is not a municipal water supply. The label should state that the water came from a “natural springwater” source.

“What types of microscopes can be used to view live organisms?”

For protozoa and algae use a compound microscope for close, detailed viewing. To observe swimming and interactions with other organisms within the culture, use a stereomicroscope. Use a compound microscope with an oil-immersion objective to view bacteria and fungus.

“What are the ingredients of Alga-Gro® media?”

Alga-Gro® media are proprietary mixtures; therefore, we do not disclose specific information about the amounts or types of ingredients they contain.

“What is the enzyme concentration?”

Enzymes do not have a specified concentration like chemicals do (e.g., 3% hydrogen peroxide) they have an activity level. The activity level varies with each enzyme. If you need to know the activity level of a particular enzyme, call our Customer Service Department (800.334.5551); and ask the representative to connect you with a technician in our Cultures Department  for this information.

“What types of organisms will I see in the Carolina™ Pond Mystery Mix or the Hay Infusion Kit?”

The number and types of organisms vary from culture to culture. Remember to always use springwater when setting up a culture; do not use tap or distilled water. It takes time for organisms to appear and become abundant, so allow at least one to 2 weeks for organisms to grow and emerge.

How to maintain algae in your classroom:

Do

  • Keep at room temperature (22° C or 72° F)
  • Loosen caps on tubes or jars and keep upright
  • Use sterile culture vessels and pipets
  • Use within 5 days of receipt or set up subcultures after 5 days to maintain longer
  • Provide correct light intensity (indirect sunlight or artificial light)
  • Read the Carolina™ Culturing Algae Manual—included with each order of algae and available separately

Don’t

  • Put in the refrigerator
  • Put in direct sunlight
  • Wash glassware in detergent
  • Keep above 30° C (86° F)—lethal to algae

Bacteria

Do I need to place my bacteria cultures in an incubator when I receive them?” The short answer is no, not every bacteria culture needs to be incubated. The temperature listed on the label is the temperature at which the culture would optimally grow if you were growing it in subculture. Most bacteria store well at room temperature and will remain viable for 3 to 4 weeks. Of course, there’s always the exception! Spirillum volutans needs to be kept at 30° C.

“Can my students get cancer from using Agrobacterium tumefaciens in the Carolina™ Plant Cancer Study Kit?”

No, they can’t. The cancer seen in susceptible plants is the result of a bacterial infection that is specific to plants, not humans. Susceptible plants include roses, grapes, apples, cherries, pecans, sunflowers, tobacco, beets, turnips, and tomatoes.

“Do I have to use a fresh, 24-hour culture of bacteria to perform a Gram stain?” Yes, you do. As the culture ages, the bacterial cell walls become more permeable to the crystal violet-iodine complex, making the cells easily decolorized. This will cause Gram-positive bacteria to stain negative.

How to Handle, Store, and Repair Microscope Slides

Carolina™ prepared microscope slides provide an essential component for the in-depth study of botany, zoology, histology, embryology, parasitology, genetics, and pathology. After receiving your slides, proper care will keep them in good condition and make them last as long as possible. In the following paragraphs, we’ll discuss the handling, storage, and repair of prepared slides.

Handling

Teach students proper slide handling and slides can be used year after year. Slides should be held by the edges, avoiding the cover glass area. Always begin viewing a slide using the microscope’s lowest magnification. This reduces the risk of contact by the microscope’s objective lens. Afterwards, switch to a higher magnification if needed.

Keep the microscope’s objective lens and other objects from coming into contact with a slide. Pressure on the cover glass can cause it to break or loosen. When finished viewing, remove the slide from the microscope and place it in its storage container. Leaving the slide on the illuminated stage for extended periods of time can cause fading and other damage.

When slides get soiled, you can clean them with soapy water or isopropyl alcohol. Do not immerse slides in water or soak them in it. This loosens the cover glass adhesive, causing the cover glass to come off and possibly ruin the slide.

Storage

To keep your prepared microscope slides in good condition, always store them in a container made for the purpose and away from heat and bright light. The ideal storage area is a cool, dark location, such as a closed cabinet in a temperature-controlled room. Stained slides naturally fade over time. Keeping them in a cool, dark location helps slow down the process.

Slides should be kept horizontal (flat) with the specimen side up. If they are stored on edge, the cover glass or specimen may shift out of position. Take care not to stack slides on top of one another or apply pressure to the cover glass.

Repair

Common problems include a broken slide or cover glass, bubbles in the mounting agent, and specimens shifted to the edge of the cover glass. If a slide or cover glass is broken, dispose of it and replace it immediately to prevent anyone from being cut. The adhesive used to attach a cover glass to a slide is applied as a liquid. As the liquid dries, it only hardens around the edges of the cover glass. With rough handling this seal can crack or loosen, allowing the liquid to ooze out. You can fix a broken seal by applying a small amount of fresh mounting media to the break. Clear nail polish sometimes works if you don’t have any mounting media handy.

Most slide repairs require some amount of skill. Often it is easier and more cost effective to replace the slide rather than to repair it.

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