Description
Intermediate – Easy to perform; requires some basic training in microbiology.
For a class of 30 students or for 6 separate teacher demonstrations. Excellent for all levels of teaching. Demonstrates the DNA extraction process of freeze-dried E. coli cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod.
The exercise helps students visualize DNA and understand some of its properties. A hot water bath, test tubes, and 70 to 95% alcohol are needed but not included. Requires 1 to 2 class periods to complete.
Includes
- 6 Freeze-Dried E. coli in Spooling Tubes (perishable item)
- 1 Bacterial Suspension Solution, 40 mL
- 1 Detergent Solution, 12 mL
- 6 Measuring Cups
- 12 Pipets
- 6 Spooling Rods
- 1 Teacher’s Manual and Reproducible Student Guide