Description
Intermediate – Easy to perform; requires some basic training in microbiology.
For a class of 30. Students cross the wild-type Sordaria strain with the mutant tan strain. Hybrid asci are produced containing 4 dark-colored and 4 light-colored ascospores. Students then use tetrad analysis to calculate the gene to centromere distance in map units for the mutant tan gene. The exercise requires about 10 to 14 days from setup of cross plates to completion. Keep fungi cultures at room temperature; do not incubate or refrigerate.
Kit and perishable components will ship separately and may arrive on different days.